Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Detection of Shiga toxin-producing Escherichia coli (STEC) O157:H7, O26, O45, O103, O111, O121, and O145, and Salmonella in retail raw ground beef using the DuPont™ BAX® system

doi: 10.3389/fcimb.2014.00081

Figure Lengend Snippet: PCR screen results for non-O157 STEC serogroup and virulence gene distribution in 308 ground beef samples analyzed .

Article Snippet: All plates were incubated at 35°C for 20–24 h. Presumptive positive colonies were tested by latex agglutination (Medina et al., ) using latex beads for non-O157 STEC obtained from Abraxis LLC (Warminster, PA) and for E. coli O157:H7 (Thermo Scientific, Waltam, MA), and then confirmed using the BAX® System Real-Time PCR assay for E. coli O157:H7 or the BAX® System Real-Time PCR STEC suite.

Techniques:

Journal:

Article Title: Polyclonal Antibodies to Glutathione S -Transferase- Verotoxin Subunit A Fusion Proteins Neutralize Verotoxins

doi: 10.1128/CDLI.9.3.687-692.2002

Figure Lengend Snippet: Characteristics of VTEC and standard strains used in neutralization studies

Article Snippet: One milliliter of culture was centrifuged at 20,000 × g for 15 min, and the supernatant was collected for a neutralization assay. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Source or strain vt genotype(s) Serotype(s) (no. of strains) Human 2 O157:H7 (3), OR:H45 (1) Cattle 1 O84:H − (1), Ont:H − (3), O121:H19 (1), O110:H16 (1) 2 O15:H − (4), O157:H7 (5), O172:H − (10), Ont:H − (3), OR:H − (3) 1, 2 O157:H7 (3), O91:H14 (1), Ont:H − (1) Variant O2:H51 (1) Pig 1, 2 Ont:H14 (1) 2, 2e Ont:H21 (1) 2e O20:H − (1), Ont:H27 (1) ATCC 43888 NT a O157:H7 ATCC 25922 (non-VTEC) NT NA b ATCC 43890 1 O157:H7 ATCC 43889 2 O157:H7 UK O157:H7 2 O157:H7 ATCC 43895 1, 2 O157:H7 WHO O157:H7 1, 2 O157:H7 S1191 2e O139 Open in a separate window a NT, non-toxin producer. b NA, not available.

Techniques: Neutralization, Variant Assay

Journal:

Article Title: Polyclonal Antibodies to Glutathione S -Transferase- Verotoxin Subunit A Fusion Proteins Neutralize Verotoxins

doi: 10.1128/CDLI.9.3.687-692.2002

Figure Lengend Snippet: Neutralization activities of the antisera. VTs produced from human and animal VTEC isolates were reacted with antisera, and the neutralization of cytotoxicity was detected.

Article Snippet: One milliliter of culture was centrifuged at 20,000 × g for 15 min, and the supernatant was collected for a neutralization assay. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Source or strain vt genotype(s) Serotype(s) (no. of strains) Human 2 O157:H7 (3), OR:H45 (1) Cattle 1 O84:H − (1), Ont:H − (3), O121:H19 (1), O110:H16 (1) 2 O15:H − (4), O157:H7 (5), O172:H − (10), Ont:H − (3), OR:H − (3) 1, 2 O157:H7 (3), O91:H14 (1), Ont:H − (1) Variant O2:H51 (1) Pig 1, 2 Ont:H14 (1) 2, 2e Ont:H21 (1) 2e O20:H − (1), Ont:H27 (1) ATCC 43888 NT a O157:H7 ATCC 25922 (non-VTEC) NT NA b ATCC 43890 1 O157:H7 ATCC 43889 2 O157:H7 UK O157:H7 2 O157:H7 ATCC 43895 1, 2 O157:H7 WHO O157:H7 1, 2 O157:H7 S1191 2e O139 Open in a separate window a NT, non-toxin producer. b NA, not available.

Techniques: Neutralization, Produced